Composite

Part:BBa_K3453174:Design

Designed by: Maeva Cherriere   Group: iGEM20_Evry_Paris-Saclay   (2020-10-24)


Grapevine VvTrnL-UAA Toehold Switch 1.1.2 with sfGFP-LVAtag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 147
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 53


Design Notes

T7 promoter (BBa_K2150031) + Grapevine VvTrnL-UAA Toehold Switch 1.1.2 (BBa_K3453073) + sfGFP-LVAtag coding sequence (BBa_K2675006) + the strong SBa_000587 Terminator (BBa_K3453000)


Source

DNA synthesis & Assembly

References